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High Cell Density Fed-Batch Production of Insecticidal ...

High Cell Density Fed-Batch Production of Insecticidal Recombinant Ribotoxin Hirsutellin A From Pichia Pastoris Microb Cell Fact . 2018 Sep 14;17(1):145. doi: 10.1186/s12934-018-0992-x.

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High cell density fed-batch production of insecticidal ...

Sep 14, 2018  High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris. Hongbo Li 1,2 Yuxian Xia 1,3 Microbial Cell Factories volume 17, Article number: 145 (2018) Cite this article

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High cell density fed-batch production of insecticidal ...

Microbial Cell Factories (2018-09-01) . High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris

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Production of lipase by high cell density fed-batch ...

Stepwise feeding fed-batch cultures were carried out to simulate an exponential feeding and to investigate the effects of specific growth rate (0.02, 0.04 and 0.08 h(-1)) on cell growth and lipase production. The highest final cell concentration obtained was 90 g l(-1) when the set point of specific growth rate (mu(set)) was 0.02 h(-1). High ...

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High cell density fed-batch production of insecticidal ...

High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris . By Hongbo Li and Yuxian Xia. Cite . BibTex; Full citation; Publisher: Springer Nature. Year: 2018. DOI identifier: 10.1186/s12934-018-0992-x. OAI identifier: Provided by: ...

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Production of lipase by high cell density fed-batch ...

Stepwise feeding fed-batch cultures were carried out to simulate an exponential feeding and to investigate the effects of specific growth rate (0.02, 0.04 and 0.08 h(-1)) on cell growth and lipase production. The highest final cell concentration obtained was 90 g l(-1) when the set point of specific growth rate (mu(set)) was 0.02 h(-1). High ...

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High cell density fed-batch production of insecticidal ...

High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris . By Hongbo Li and Yuxian Xia. Cite . BibTex; Full citation; Publisher: Springer Nature. Year: 2018. DOI identifier: 10.1186/s12934-018-0992-x. OAI identifier: Provided by: ...

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High-cell-density fed-batch cultivation of the ...

Vigorous mixing was required to sustain aerobic conditions during high-cell-density cultivation. This was complicated by culture viscosity, which resulted from the production of viscous extracellular polysaccharides. These may present a problem for large-scale industrial production of DHA.

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Avoiding overfeeding in high cell density fed-batch ...

Avoiding overfeeding in high cell density fed-batch cultures of E. coli during the production of heterologous proteins J Biotechnol . 2014 Dec 20;192 Pt A:146-53. doi: 10.1016/j.jbiotec.2014.09.002.

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Production of nattokinase by high cell density fed-batch ...

Feb 20, 2011  Bacillus subtilis was cultivated to high cell density for nattokinase production by pH-stat fed-batch culture. A concentrated mixture solution of glucose and peptone was automatically added by acid-supplying pump when culture pH rose above high limit. Effect of the ratio of glucose to peptone in feeding solution was investigated on cell growth and nattokinase production by changing the ratio ...

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(PDF) High-cell-density fed-batch cultivation of the ...

High-cell-density fed-batch cultivation of the docosahexaenoic acid producing marine algaCrypthecodinium cohnii. Biotechnology and Bioengineering, 2003. Lolke Sijtsma. Download PDF. Download Full PDF Package. This paper. A short summary of this

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Developments in high cell density and high productivity ...

Apr 01, 1994  YEE L, BLANCH HW: Recombinant Trypsin Production in High Cell Density Fed-Batch Cultures in Escherichia coll. Blotechnol Bioeng 1993, 41:781-790. An optimized defined medium is used to grow E. colito 92 g 1-1 DCW, with production of the serine protease, rat anionic trypsin, to a level of 56mg1-1 in the periplasm.

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High Cell Density Fermentation-Biologicscorp

High Cell Density Fermentation Technology High cell density fermentation by fed-batch strategies is one of the most cost-effective means of achieving high yields for the production of large scale recombinant proteins in the bio-industry. In fed-batch cultures, cell mass and productivity are maximized by adjusting culture conditions, including temperature and pH, the composition of the []

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Recombinant Protein Expression in High Cell Density Fed ...

Dec 01, 1992  Whereas cell concentrations of 5–10 grams dry cell weight per liter (gDCW/1) are typical of batch cultures, fed-batch techniques can be used to achieve concentrations greater than 50 gDCW/1.

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夏玉先 教授-生命科学科学学院

Jul 15, 2017  [28] Li Hongbo, Xia Yuxian. (2018) High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris. MICROBIAL CELL FACTORIES, 17: 145. [29] Li Hongbo, Xia Yuxian. (2018) High-level expression and purification of active scorpion long-chain neurotoxin Bj alpha IT from Pichia pastoris.

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High-level expression and purification of active scorpion ...

Jul 30, 2018  High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris. Li H, Xia Y. Microb Cell Fact, 17(1):145, 14 Sep 2018 Cited by: 0 articles PMID: 30342541 PMCID: PMC6195745. Free to read use

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[PDF] Mass Cultivation of Bacillus thuringiensis var ...

Batch and fed-batch cultivation of Bacillus thurillgiellsis var. kurstaki was carried out to achieve high cell and spore density with maximum insecticidal activity. Cell cultivation was done in fully equipped fermentors under controlled conditions at different feed rates. Increased cell mass, high spore counts and improved insecticidal activity were achieved with fed-batch cultivation system.

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Quantitative physiology of Pichia pastoris during glucose ...

To investigate the impact of recombinant protein production and high-cell density fermentation on the metabolism of P. pastoris, we used 13 C-tracer-based metabolic flux analysis in batch and fed-batch

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Enhanced production of human mini-proinsulin in fed-batch ...

The fermentative production of this small fusion mini-proinsulin may be of great advantage in enhancing the yield of human insulin. To find an optimum induction strategy, effects of various key cultivation variables on the mini-proinsulin production are examined in high cell density fed-batch cultures.

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High cell density fed-batch production of insecticidal ...

Conclusions This is the first report of rHtA expression in P. pastoris. The rHtA was expressed at a high level under high-cell-density fed-batch fermentation and was efficiently purified using a two-step purification method. Purified rHtA exhibited thermal and protease stability, as

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High cell density fed-batch production of insecticidal ...

High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris . By Hongbo Li and Yuxian Xia. Cite . BibTex; Full citation; Publisher: Springer Nature. Year: 2018. DOI identifier: 10.1186/s12934-018-0992-x. OAI identifier: Provided by: ...

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High cell density fed-batch fermentation for the ...

Apr 15, 2014  A high cell density production process typically involves fed-batch fermentation . The feeding is controlled to ensure that the substrate concentration does not build up to an inhibitory level [12] ,

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High‐cell‐density fed‐batch cultivation of the ...

Jan 10, 2003  Vigorous mixing was required to sustain aerobic conditions during high‐cell‐density cultivation. This was complicated by culture viscosity, which resulted from the production of viscous extracellular polysaccharides. These may present a problem for large‐scale industrial production

Get price

Recombinant Protein Expression in High Cell Density Fed ...

Dec 01, 1992  Whereas cell concentrations of 5–10 grams dry cell weight per liter (gDCW/1) are typical of batch cultures, fed-batch techniques can be used to achieve concentrations greater than 50 gDCW/1.

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High cell density culture of the diatom Nitzschia laevis ...

Jul 01, 2002  A fed-batch process was developed for high cell density culture of the diatom Nitzschia laevis for enhanced production of eicosapentaenoic acid (EPA). Firstly, among the various medium components, glucose (Glu) was identified as the limiting substrate while nitrate (NO 3 −), tryptone (Tr) and yeast extract (Ye) were found to promote cell growth by enhancing specific growth rate.

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High Cell Density Fermentation of Saccharomyces

High Cell Density Fermentation of Saccharomyces cerevisiae JUL3 in Fed-batch Culture for the Production of β-Glucan Young-Hwan Kim, Seong Woo Kang, Jong Ho Lee, Hyo-lhl Chang*, Cheol-Won Yun*, Hyun-Dong Paik**, Chang-Won Kang**, and Seung Wook Kim† Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea

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(PDF) High-cell-density fed-batch cultivation of the ...

High-cell-density fed-batch cultivation of the docosahexaenoic acid producing marine algaCrypthecodinium cohnii. Biotechnology and Bioengineering, 2003. Lolke Sijtsma. Download PDF. Download Full PDF Package. This paper. A short summary of this

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High Cell Density Fed-batch Fermentation for the ...

High Cell Density Fed-batch Fermentation for the Production of Recombinant E. coli K-12 Ghost Vaccine against Streptococcal Disease [2011] Park, S.J., Pukyong National University, Busan, Republic of Korea; Lee, S.J., Pukyong ...

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Developments in high cell density and high productivity ...

Apr 01, 1994  YEE L, BLANCH HW: Recombinant Trypsin Production in High Cell Density Fed-Batch Cultures in Escherichia coll. Blotechnol Bioeng 1993, 41:781-790. An optimized defined medium is used to grow E. colito 92 g 1-1 DCW, with production of the serine protease, rat anionic trypsin, to a level of 56mg1-1 in the periplasm.

Get price

Enhanced Production of Human Mini‐Proinsulin in Fed‐Batch ...

Sep 04, 2008  The fermentative production of this small fusion mini-proinsulin may be of great advantage in enhancing the yield of human insulin. To find an optimum induction strategy, effects of various key cultivation variables on the mini-proinsulin production are examined in high cell density fed-batch cultures.

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Production of extracellular inulinase in high-cell-density ...

Production of extracellular inulinase in high-cell-density fed-batch cultures of Kluyveromyces marxianus. M. Hensing 1, H. Vrouwenvelder 1, C. Hellinga 2, R. Baartmans 1 H. van Dijken 1 Applied Microbiology and Biotechnology volume 42, pages 516–521 (1994)Cite this article

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High Cell Density Fermentation-Biologicscorp

High Cell Density Fermentation Technology High cell density fermentation by fed-batch strategies is one of the most cost-effective means of achieving high yields for the production of large scale recombinant proteins in the bio-industry. In fed-batch cultures, cell mass and productivity are maximized by adjusting culture conditions, including temperature and pH, the composition of the []

Get price

High cell density fed-batch fermentations for lipase ...

Mar 29, 2013  Kim BS, Hou CT (2006) Production of lipase by high cell density fed-batch culture of Candida cylindracea. Bioprocess Biosyst Eng 29:59–64. CAS Google Scholar 109. Li C-Y, Cheng C-Y, Chen T-L (2004) Fed-batch production of lipase by Acinetobacter radioresistens using Tween 80 as the carbon source. Biochem Eng J 19:25–31

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